Molecular Cloning and Functional Expression of cDNAs Encoding Oxidosqualene Cyclases from Costus speciosus.
نویسندگان
چکیده
منابع مشابه
Cloning of oxidosqualene cyclases from Maytenus ilicifolia for synthetic biology
Material and methods For this purpose, we adopted the strategy of RT-PCR using degenerated primers obtained from sequences of known plant OSC genes. Total RNA was extracted from leaves of M. ilicifolia and used in the synthesis of cDNA. The PCR amplification of core fragments of OSC genes was performed with partially degenerated primers designed to anneal to highly conserved regions among OSC g...
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Betula platyphylla var. japonica is a rich source of triterpenoid as it contains dammarane type triterpenes in the leaves, and lupane type and oleanane type triterpenes in the bark. Four oxidosqualene cyclase cDNAs (BPX, BPX2, BPW and BPY) were cloned by homology based PCR methods from cell suspension cultures of B. platyphylla var. japonica. Open reading frames consisting of 2274, 2304, 2268 a...
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Costus speciosus Koen. (Keu, Crape ginger), an Indian ornamental plant, has long been medicinally used in traditional systems of medicine. The plant has been found to possess diverse number of pharmacological activities. The present article gives an account of updated information on its phytochemical and pharmacological properties. The review reveals that wide numbers of phytochemical constitue...
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A cDNA encoding rat oxidosqualene lanosterol-cyclase [lanosterol synthase; (S)-2,3-epoxysqualene mutase (cyclizing, lanosterol-forming), EC 5.4.99.7] was cloned and sequenced by a combination of PCR amplification, using primers based on internal amino acid sequence of the purified enzyme, and cDNA library screening by oligonucleotide hybridization. An open reading frame of 2199 bp encodes a M(r...
متن کاملCostus speciosus: An Important Medicinal Plant
Costus speciosus is an important medicinal and ornamental plant used to cure different diseases. The plant has been found to possess many pharmacological activities such as antibacterial, antifungal, anticholineesterase, antioxidant, antihyperglycemic, antiinflammatory, analgesic, antipyretic, antidiuretic, larvicidal, antistress and estrogenic activity. The rhizomes of Costus speciosus are bit...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2002
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.25.477